منابع مشابه
An automated alkaline phosphatase assay with phenolphthalein monophosphate as substrate.
The manual procedure of Babson et al. (1) for the determination of serum alkaline phosphatase has been automated. The assay is based on the colorimetric determination of phenolphthalein formed on hydrolysis of phenolphthalein monophosphate. The procedure utilizes 0.16 ml. of serum without dialysis. Blanks are required only for turbid sera. Results are compared with those obtained by the manual ...
متن کاملPhenolphthalein monophosphate as a substrate for serum alkaline phosphatase. An appraisal.
A rapid and precise procedure for serum alkaline phosphatase employs phenolphthalein monophosphate as the substrate. This compound is readily hydrolyzed and directly provides a chromogen for colorimetric determination. The color produced is linear with respect to both enzyme concentration and incubation time and is stable. Reagent and serum blanks are negligible. Various factors which affect al...
متن کاملalpha-Naphthyl phosphate: the preferred substrate for acid phosphatase.
± 2 S , which equals ± 9 mg of calcium per liter) may range from 86 to 104 mg/L! This means that a patient whose whole serum calcium is just about at the mid-point of the reference range when measured by atomic absorption may be classified (within the 95% confidence interval) as either borderline hypocalcemic or borderline hypercalcemic when the kit is used. If atomic absorption is considered t...
متن کاملAutoAnalyzer assay for serum alkaline phosphatase activity, with sodium thymolphthalein monophosphate as substrate.
An automated method is described for determining alkaline phosphatase activity in serum, with use of sodium thymolphthalein monophosphate as the substrate. The system makes use of standard AutoAnalyzer components, has a simple flow diagram, and does not involve dialysis. The method has good precision, and the results correlate well with those from the manual method of which it is an adaptation.
متن کاملImproved determination of prostatic acid phosphatase (sodium thymolphthalein monophosphate substrate).
We have modified a previously described method for determining acid phosphatase, with thymolphthalein monophosphate as substrate, to increase its sensitivity. We assessed effects of serum on variables influencing acid phosphatase activity as measured by this method. The method is shown to be not completely specific for prostatic acid phosphatase. The importance of standardizing methodology in m...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1945
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)52801-0